Benoit kornmann eth. Framework and implementation. , 2021; Kornmann ETH Z€urich, Otto-Stern-Weg 3, 8093 Z €urich, Switzerland Tel: +41 44 633 2856 E-mail: arun. Benoit Kornmann studies membrane contact sites, how intracellular organization impinges on organelle function and how lipid molecules are distributed among the many membranes of a eukaryotic cell. ch Apr 11, 2016 · During mitosis, cells undergo massive deformation and reorganization, impacting on all cellular structures. The Kornmann Lab studies interorganelle communication and is located at the Biochemistry Department of Oxford University, . cerevisiae ([Michel et al. This plasticity ensures the proper distribution of the metabolism, and the proper inheritance of functional organelles. Previous affiliations of Benoît Kornmann include ETH Zurich & École Polytechnique Fédérale de Lausanne. Mar 11, 2019 · We thank Andrea Aloia and Werner Kovacs (ETH Zurich) for support with the Seahorse measurements. Mechanobiology is the study of the interaction between mechanical stimuli and biological systems. was supported by a postdoctoral fellowship from the National Cancer Institute (T32 CA009523). 5. R. Apr 15, 2016 · Affiliations 1 Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland. They provide an appropriate milieu for the catalysis of specific biochemical reactions. John Peter, Institute of Biochemistry, ETH Zürich, Otto-Stern-Weg 3, 8093 Zürich, Switzerland Aug 16, 2019 · Mechanobiology studies from the last decades have brought significant insights into many domains of biological research, from development to cellular signaling. John Peter currently works at the Institute of Biochemistry, ETH Zurich. unveils one potential role of the ER-droplet tether: Liquid droplets undergo division at sites where ER tubules are present. Correspondence. We previously established SAturated Transposon Analysis in Yeast (SATAY) to uncover genes necessary for growth in any condition in S. ox. Associate Professor at University of Oxford · Experience: University of Oxford · Education: Oct 29, 2018 · 1 Institute of Biochemistry, ETH Zurich, 8093 Zürich, Switzerland qian. Raymond T. His most recent publication is 'Vps13-Mcp1 interact at Benoit Kornmann is Associate Professor at the Department of Biochemistry, University of Oxford, and fellow of St Hugh’s College. During cell cycle Jul 13, 2012 · Two talks, one by Will Prinz (The National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, USA) and another by Benoît Kornmann (ETH Zürich, Switzerland), showed how a protein complex that tethers the mitochondria to the endoplasmic reticulum is necessary for an appropriate lipid balance in the Jun 6, 2021 · Information on the co-evolution of amino acid pairs in a protein can be used for endeavors such as protein engineering, mutation design, and structure prediction. When provided with a protein sequence, we derive its embedding using the pre-trained ESM-1b model31. The approach, based on transposon mutagenesis followed by deep sequencing, takes advantage of the recent ease of using deep sequencing to circumvent most of the disadvantages of previous yeast screening Benoît Kornmann is an academic researcher from University of Oxford. Consequently, understanding protein function is of paramount importance for comprehending health, disease, evolution, and the functioning of living organisms at the molecular level 1 – 3. This parallels the previous discovery by the same laboratory that the sites of division for two membrane-bound organelles, the endosomes and the mitochondria (9, 10), are also characterized by the nearby presence and perhaps Membrane-bound organelles are a wonderful evolutionary acquisition of the eukaryotic cell, allowing the segregation of sometimes incompatible biochemical reactions into specific compartments with tailored microenvironments. kornmann@bc Sep 9, 2015 · Sequencing was done at the Functional Genomics Center Zürich. ch Abbreviations used in this paper: ERMES, ER–mitochondria encounter structure; MDM, mitochondrial distribution and morphology May 1, 2010 · While ERMES plays a crucial role during mitophagy in yeast (Böckler and Westermann, 2014), most of its subunits seem to be absent from mammalian genomes (Aoyama-Ishiwatari et al. European Molecular Biology Organization ALTF 1115-2014 to Qian Feng. View benoit kornmann’s profile on LinkedIn, a professional community of 1 billion members. Françoise Stutz Jan 31, 2020 · An interesting observation by Lee et al. ETH Zürich - Institute of Biochemistry. Oct 10, 2017 · Imaging was done at the ETH Zürich ScopeM facility. Nov 9, 2021 · Mitochondria and the complex endomembrane system are hallmarks of eukaryotic cells. SCJ Helle, G Kanfer, K Kolar, A Lang, AH Michel, B Kornmann. Organelles are separate yet interdependent units of the eukaryotic cells. To date, it has been difficult to manipulate organelle structures within single live cells. benoit. The leri web-server takes a single sequence or a multiple sequence alignment (MSA) as input to infer evolutionary couplings and residue communities that could be determinants of functional specificity (Figure S1). Jul 15, 2011 · 1Present address: ETH Zürich, Institute of Biochemistry, Zürich CH-8093, Switzerland. This complex topology, however, makes a permanent crosstalk between the organelles a necessity for the coo … Aug 16, 2019 · 1. May 8, 2017 · Benoît Kornmann Institute of Biochemistry, ETH Zurich, Zurich, Switzerland benoit. Coordinated membrane biogenesis of different organelles necessitates lipid transport from their site of synthesis to their destination. The author has contributed to research in topics: Endoplasmic reticulum & Organelle. E-mail: benoit. group vCard (vcf) my page on the Biochemistry Department website my page on St-Hugh's process, BP, cellular component, CC, and molecular function, MF), to proteins. Mitochondria, in particular, are highly dynamic organelles, which constantly undergo events of fission, fusion and cytoskeleton-based transport. On the other hand, the compartimentalization of the cell also creates the need for ultrastructural organization of the cell […] Mar 23, 2022 · We thank Maximilian Mittelviefhaus (ETH Zurich), Patrick Frederix (Nanosurf), Pablo Dörig, and Dario Ossola (Cytosurge AG, Switzerland) for their support, as well as Stephen Wheeler (ETH Zurich) and the personnel of ETH ScopeM facility and the Functional Genomics Center Zurich for technical assistance. A standout feature of eukaryotic cells is the presence of organelles with distinct chemical compositions and physical properties, which aid in the accomplishment of specialized metabolic tasks. Kornmann, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK Tel: +44 1865 613298 E-mail: benoit. This study describes a novel microfluidic device that allows the extraction of organelles, including mitochondria, from viable cells and their reintroduction into recipient host cells. ch. Kornmann. Jul 1, 2020 · ETH Zürich - Institute of Biochemistry. feng@bc. uk 🌐 www. 1042/BST20110758 Abstract Interests: I study the ultrastructural organization of the cell and the biology of organelles. , 2014; Hönscher et al. Defects in ERMES can, however, be bypassed by point mutations in the endosomal protein Vps13 or … Benoit Kornmann studies membrane contact sites, how intracellular organization impinges on organelle function and how lipid molecules are distributed among the many membranes of a eukaryotic cell. Thus, mitochondria adapt to the environment by sensing and responding to biomechanical cues. For each gene, a score is computed as follows: the longest interval between transposon n and transposon n + 5, multiplied by the total number of transposons mapping to that gene, divided by the gene length to the power of 1. Feb 15, 2017 · Chorea-Acanthocytosis (ChAc) is a rare hereditary neurological disorder characterized by abnormal movements, red blood cell pathology, and progressive neurodegeneration. Suhandynata. , 2009; Elbaz-Alon et al. How th … Arun T John Peter 1 , Benoit Kornmann 2 ETH Zürich, Switzerland. Introduction. University of California, San Diego (UCSD) - Department of Cellular & Molecular Medicine. 1. Organization and function of membrane contact sites. Huilin Zhou. We also thank all members of the Kornmann lab for providing comments on the manuscript. Such communication involves the formation of membrane contact sites between adjacent organelles, allowing privileged exchange of metabolites and information. Benoit Kornmann [email protected] Department of Biochemistry, University of Oxford, UK. Arun T. SAturated Transposon Analysis in Yeast (SATAY) all … Sep 14, 2015 · Correspondence to Benoît Kornmann: benoit. Nov 11, 2021 · The Kornmann lab is supported by a grant from the Swiss National Science Foundation (31003A_179549) and the Wellcome Trust (214291/Z/18/Z). Here we report a method that captures significant determinants of proteins using estimated co-evolution information to identify networks … Benoit Kornmann studies membrane contact sites, how intracellular organization impinges on organelle function and how lipid molecules are distributed among the many membranes of a eukaryotic cell. Eukaryotic cells are compartmentalized into membrane-bound organelles, each of which performs distinct biochemical tasks. 3 Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, Netherlands. ) and Benoît Kornmann (ETH Zurich, Zurich, Switzerland) explains how defects in ER–mitochondrial contacts can be bypassed by other organelle contact sites (Lang et al. ch Benoit. ). PMID: 38268324 Affiliation 1 Institute of Biochemistry, ETH Zürich, 8093 Zürich, Switzerland. Proteins bind to other molecules to facilitate nearly all essential biological activities. volkov@tudelft. 1242/jcs. 5 Institute of Biochemistry, ETH Zurich, ZH 8093 Zurich, Switzerland benoit. T. ac. We developed a FluidFM-based approach to extract, inject and transplant organelles from and into living cells with subcellular spatial resolution. The exact role of Miro in these functions is unclear but involves binding to a variety of client proteins. Jan 24, 2024 · Corresponding Author. 218479. Microscopy was performed at the Scientific Center for Optical and Electron Microscopy of the ETH Zurich. nl. Microscopy analysis was carried out at the ETH Zürich ScopeM facility, and lipidomic measurements and Illumina sequencing at the Functional Genomics Center Zurich (FGCZ). Cells in the body are subjected to diverse mechanical stresses, including shear stress by blood flow, tensional stress due to muscle stretching, compression by the skeleton and more []. Sep 9, 2017 · We are grateful to the members of Kornmann group for discussions, Richard McIntosh (University of Colorado) and Louis Reese (Delft University of Technology) for critical reading of the manuscript, Arkady Fradkov (ZAO Evrogen, Moscow, Russia) for excellent technical assistance with the cloning of CENP-F fragments, and Martin Jinek (University of May 8, 2017 · Yeast is a powerful model for systems genetics. Kornmann et al. Imaging was performed at the ETH Zürich ScopeM facility. ch v. Arun does research in Molecular Biology and Cell Biology. The intracellular environment of eukaryotic cells is highly complex and compact. Nederlandse Organisatie voor Wetenschappelijk Onderzoek Rubicon 825. Miro proteins are universally conserved mitochondrial calcium-binding GTPases that regulate a multitude of mitochondrial processes, including transport, clearance, and lipid trafficking. Abstract. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1833 (11), The Kornmann Laboratory studies the ultrastructural organization of the cell and the biology of organelles. kornmann. We present an improved version leveraging homologous recombination to increase Jun 6, 2021 · 2. DOI: 10. , 2014; Lahiri et al. ch Competing interests The authors declare that no May 8, 2017 · Top, algorithm to detect essential protein domains. , 2009) have been described previously. Dec 6, 2019 · The analysis of large-scale transposon mutant libraries is becoming a method of choice for functional genomics in bacteria and fungi. The author has an hindex of 25, co-authored 62 publications receiving 6411 citations. 2 Laboratory of Biosensors and Bioelectronics, Institute for Biomedical Engineering, ETH Zurich, Zurich, Switzerland. Benoît KORNMANN | Cited by 7,488 | of ETH Zurich, Zürich (ETH Zürich) | Read 105 publications | Contact Benoît KORNMANN Mar 23, 2022 · Mitochondria and the complex endomembrane system are hallmarks of eukaryotic cells, but it has proved difficult to manipulate organelle structures within single live cells. , 2015) highlights the need for coordinated Apr 1, 2012 · The essential reactions in eukaryotic cells are compartmentalized in membrane-bound organelles, which allow the segregation of incompatible biological processes with tailored microenvironments [1,25]. Sep 1, 2017 · 4 Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, 119991 Moscow, Russia benoit. T. Benoît KORNMANN | Cited by 7,751 | of ETH Zurich, Zürich (ETH Zürich) | Read 109 publications | Contact Benoît KORNMANN 2019. P. However, mechano-regulation of subcellular components, especially membranous organelles, are only beginning to be unraveled. Affiliation 1 Institute ETH Zurich, 8093 Zurich, Switzerland. A. 023 to Qian Feng. The ERMES complex is a well-characterized struc-ture that tethers the ER to the outer mitochondrial membrane (OMM; Michel and Kornmann, 2012). We present a versatile, time- and labor-efficient method to functionally explore the <i>Saccharomyces cerevisiae</i> genome using saturated transposon mutagenesis coupled to high-throughput sequencing. Nov 9, 2017 · We propose that MFF, owing to affinity for narrow mitochondria, acts as a membrane-bound force sensor to recruit the fission machinery to mechanically strained sites. The approach enabled the transfer of controlled quantities of Feb 21, 2022 · We are thankful to members of the Peter and Kornmann laboratories for discussions and helpful suggestions. kornmann@bioch. The core–ERMES com-plex consists of the ER protein Mmm1 (mitochondria morphol-ogy maintenance 1), the OMM proteins Mdm10 and Mdm34, Benoît Kornmann, PhD Associate Professor, Department of Biochemistry Tutorial Fellow, St-Hugh's College South Parks Road, Oxford OX1 3QU ☎ +44 (0) 1865 613298 benoit. 14. Aug 4, 2024 · Understanding protein function is pivotal in comprehending the intricate mechanisms that underlie many crucial biological activities, with far-reaching implications in the fields of medicine, biotechnology, and drug development. The Kornmann Laboratory studies the ultrastructural organization of the cell and the biology of organelles. S. john@bc. The distinct activities of organelles depend on the proper function of their membranes. This work was supported by the Swiss National Science Foundation (grant #PP00P3_133651) and the European Research Council (ERC-2013-StG 337906-OrgaNet) to B. We study in particular how mitochondria integrate with the rest of cellular activity Nov 9, 2017 · 1 Institute of Biochemistry, ETH Zurich, Zurich, Switzerland. ; 2 Institute of Biochemistry, ETH Zurich, 8093 Zurich, Switzerland benoit. Using a synthetic protein designed to artificially tether the ER (endoplasmic reticulum) to mitochondria, we have Mar 3, 2018 · Introduction. We thank Sean Munro for plasmids. Several factors have been proposed to participate in lipid distribution, but … Benoît Kornmann is currently working as professor, Department of Biology of ETH Zurich. In this paper, … Oct 2, 2017 · Membrane contact sites between endoplasmic reticulum (ER) and mitochondria, mediated by the ER-mitochondria encounter structure (ERMES) complex, are critical for mitochondrial homeostasis and cell growth. 2 To whom correspondence may be addressed. Little is understood of the pathogenesis of ChAc and related disorders (collectively . However, the recent identification of direct physical contacts between the membranes of two organelles (Kornmann et al. Kornmann@bc. Aug 4, 2024 · Introduction. Benoit Kornmann is an expert in yeast genetics, membrane biology, organelle dynamics and signalling. ethz. biol. Subsequently, we Jul 13, 2012 · Two talks, one by Will Prinz (The National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, USA) and another by Benoît Kornmann (ETH Zürich, Switzerland), showed how a protein complex that tethers the mitochondria to the endoplasmic reticulum is necessary for an appropriate lipid balance in the Mar 3, 2018 · The Minisymposium on “Organelle Morphogenesis, Targeting, and Distribution” covered mechanisms governing organelle identity, explored organellar contacts and interactions with the cytoskeleton, examined new data suggesting how cells and organelles are intrinsically capable of “measuring,” and finally considered how some of these structures are remodeled during cell division. We developed a FluidFM-based approach to extract, inject, and transplant organelles from and into living cells with subcellu … Mar 21, 2012 · Cellular organelles need to communicate in order to co-ordinate homoeostasis of the compartmentalized eukaryotic cell. uk (Received 18 October 2023, revised 11 December 2023, accepted 4 Aug 4, 2024 · Understanding protein function is pivotal in comprehending the intricate mechanisms that underlie many crucial biological activities, with far-reaching implications in the fields of medicine Mar 23, 2022 · Mitochondria and the complex endomembrane system are hallmarks of eukaryotic cells. Research. kornmann@bc. ch Agnès H Michel 1 , Benoît Kornmann. ch and B. PMID: 22435828 DOI: 10. This algorithm is implemented in Source code 2. PMID: 30373893. Research Interest Benoît Kornmann research interest include: Biochemistry. This work was supported by Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (grant PP00P3_133651) and the H2020 European Research Council (ERC-2013-StG 337906-OrgaNet) to B. 2 Department of Biochemistry, University of Oxford, UK. On the flip side, these isolating membranes that crowd the interior of the c … May 8, 2017 · The manuscript by Michel, Kimmig & Kornmann describes an innovative technology to rapidly and efficiently screen yeast for causal genomic loci. However, more than 200 million proteins remain uncharacterized, and com … Sep 14, 2015 · In the September 14th edition of biobytes, Patrick Caswell (University of Manchester, Manchester, UK) describes how actin spikes drive 3D cell migration (Paul et al. Imaging was done at the ETH Zürich ScopeM facility. , 2017][1]). , 2014; Murley et al. Nov 9, 2017 · Eidgenössische Technische Hochschule Zürich ETH-16 13-2 to Sebastian Carsten Johannes Helle, Benoît Kornmann. 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